Evaluation of commercially available small RNASeq library preparation kits using low input RNA

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Evaluation of commercially available RNA amplification kits for RNA sequencing using very low input amounts of total RNA.

This article includes supplemental data. Please visit http://www.fasebj.org to obtain this information.Multiple recent publications on RNA sequencing (RNA-seq) have demonstrated the power of next-generation sequencing technologies in whole-transcriptome analysis. Vendor-specific protocols used for RNA library construction often require at least 100 ng total RNA. However, under certain condition...

متن کامل

Evaluation of commercially available Helicobacter pylori serology kits: a review.

s on disk. Marathon Multimedia, Northfield, Minn.13. Cammarota, G., G. Fedeli, M. Montalto, et al. 1994. The use of Helicobacterpylori serology in diagnosis and treatment monitoring. In Proceedings of the EHPSG VIIth International Conference.14. Chen, T. S., F. Y. Chang, and S. D. Lee. 1997. Serodiagnosis of Helicobacterpylori infection: comparison and correlation between enzyme...

متن کامل

Comparison of four commercially available cAMP kits.

In this study four commercially available cAMP kits were compared and shown to differ with respect to reliability, sensitivity, simplicity and expiration time. It is not possible to recommend a particular kit, because the choice depends on the demands of the investigator, which, of course, may vary. However, in our opinion, one of the kits was inferior to the others.

متن کامل

A comparison of commercially-available automated and manual extraction kits for the isolation of total RNA from small tissue samples

BACKGROUND This study compared the performance of five commercially available kits in extracting total RNA from small eukaryotic tissue samples (<15 mg). Total RNA was isolated from fathead minnow (Pimephales promelas) tissues (spleen, blood, kidney, embryo, and larvae) using the Qiagen RNeasy® Plus Mini, Qiagen RNeasy® Plus Universal, Promega Maxwell® 16 LEV simplyRNA, Ambion MagMAX™-96 and Pr...

متن کامل

RNAseq by Total RNA Library Identifies Additional RNAs Compared to Poly(A) RNA Library

The most popular RNA library used for RNA sequencing is the poly(A) captured RNA library. This library captures RNA based on the presence of poly(A) tails at the 3' end. Another type of RNA library for RNA sequencing is the total RNA library which differs from the poly(A) library by capture method and price. The total RNA library costs more and its capture of RNA is not dependent on the presenc...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: BMC Genomics

سال: 2018

ISSN: 1471-2164

DOI: 10.1186/s12864-018-4726-6